Human induced pluripotent stem cells (hiPSCs) represent an almost limitless source of cells for biomedical applications including cardiomyocytes (CMs), the most predominant cell type in the human heart. iBB researchers have established an efficient and robust 3D platform for CM production from hiPSCs and studied the impact of 3D culture on CM differentiation and maturation compared with a 2D monolayer culture. It was found that CMs mature earlier and show an improved communication system in this 3D environment which was suggested to be responsible for a higher structural and functional maturation. This novel 3D culture platform and the CMs obtained can be used for disease modelling, drug screening and cardiotoxicity tests. The results were published in Scientific Reports.